Spurs’ duo Kieran Trippier and Harry Winks along with Southampton pair Nathan Redmond and James Ward-Prowse have been omitted after being included in the original 27-man interim squad named by manager Gareth Southgate earlier this month.
England face the Netherlands in the semi-finals in Guimaraes on 6 June.
Hosts Portugal play Switzerland in the other semi-final on 5 June in Porto. The third-place play-off and final are on 9 June.
Trent Alexander-Arnold (Liverpool), Dele Alli (Tottenham Hotspur), Ross Barkley (Chelsea), Jack Butland (Stoke City), Ben Chilwell (Leicester City), Fabian Delph (Manchester City), Eric Dier (Tottenham Hotspur), Joe Gomez (Liverpool), Tom Heaton (Burnley), Jordan Henderson (Liverpool), Harry Kane (Tottenham Hotspur), Michael Keane (Everton), Jesse Lingard (Manchester United), Harry Maguire (Leicester City), Jordan Pickford (Everton), Marcus Rashford (Manchester United), Declan Rice (West Ham United), Danny Rose (Tottenham Hotspur), Jadon Sancho (Borussia Dortmund), Raheem Sterling (Manchester City), John Stones (Manchester City), Kyle Walker (Manchester City), Callum Wilson (AFC Bournemouth)