John Stones, Kyle Walker, Raheem Sterling and Phil Foden have all been included in a squad that will be trimmed back to 26 on June 1 after the Champions League and Europa League finals later this week.
After City's superb campaign, the quartet will all be hopeful of making the final cut next week.
Dean Henderson (Manchester United), Sam Johnstone (West Brom), Jordan Pickford (Everton), Aaron Ramsdale (Sheffield United).
Trent Alexander-Arnold (Liverpool), Ben Chilwell (Chelsea), Conor Coady (Wolves), Ben Godfrey (Everton), Reece James (Chelsea), Harry Maguire (Manchester United), Tyrone Mings (Aston Villa), Luke Shaw (Manchester United), John Stones (Manchester City), Kieran Trippier (Atletico Madrid), Kyle Walker (Manchester City), Ben White (Brighton).
Jude Bellingham (Borussia Dortmund), Phil Foden (Manchester City), Jack Grealish (Aston Villa), Jordan Henderson (Liverpool), Jesse Lingard (Manchester United), Mason Mount (Chelsea), Kalvin Phillips (Leeds), Declan Rice (West Ham), Bukayo Saka (Arsenal) Jadon Sancho (Borussia Dortmund), James Ward-Prowse (Southampton).
Dominic Calvert-Lewin (Everton), Mason Greenwood (Manchester United), Harry Kane (Tottenham), Marcus Rashford (Manchester United), Raheem Sterling (Manchester City), Ollie Watkins (Aston Villa)..
Ahead of naming his final #EURO2020 squad next week, Gareth Southgate has selected 33 players to join up with the #ThreeLions from this weekend as our preparations for this summer's tournament get under way.— England (@England) May 25, 2021