England begin their Euro 2024 qualifying campaign with a tough trip to Italy on March 23, before Ukraine travel to Wembley three days later.
Malta and North Macedonia are also in Group C with the Three Lions facing them in a double-header in June.
All five City players have kept their places in Southgate’s squad from the World Cup when England reached the quarter-finals before losing to France.
The ten group winners and the ten group runners-up qualify directly for the final tournament along with hosts Germany with play-offs for the remaining spots in March 2024.
Defenders: Ben Chilwell (Chelsea), Eric Dier (Tottenham Hotspur), Marc Guehi (Crystal Palace), Reece James (Chelsea), Harry Maguire (Manchester United), Luke Shaw (Manchester United), John Stones (Manchester City), Kieran Trippier (Newcastle United), Kyle Walker (Manchester City)
Midfielders: Jude Bellingham (Borussia Dortmund), Conor Gallagher (Chelsea), Jordan Henderson (Liverpool), James Maddison (Leicester City), Mason Mount (Chelsea), Kalvin Phillips (Manchester City), Declan Rice (West Ham United)