The newly-promoted Dutch side were soundly beaten 4-1 by SVB Vitesse, with five City youngsters featuring.
For Ambrose it was a seminal moment with the French youngster seeing much of last season ruined by injury – it was also his first ever senior goal.
Angelino, Paolo Fernandes and Thomas Agyepong also played and the quintet will be hoping for better days in the coming weeks.
Staying in Holland, Isaac Buckley-Ricketts came on after 75 minutes for FC Twente who lost 2-1 to Feyenoord.
In the Premier League, Joe Hart had a miserable return to Manchester after West Ham were soundly beaten by United at Old Trafford.
In the Championship, Bersant Celina remained on the bench as Ipswich Town came from behind to win 2-1 at Barnsley - the first time the Tractor Boys have started with back-to-back wins since 2002/03.
Angus Gunn endured a miserable afternoon on goal for Norwich who were beaten 3-1 by Sunderland at Carrow Road.
Dropping down to League One and Rodney Kongolo made his second substitute appearance for Doncaster Rovers at the weekend.
The Dutch teenager came on for Doncaster legend James Coppinger after 84 minutes as Darren Ferguson’s side beat Blackburn Rovers 3-1 at Ewood Park.
Finally this week, Ashley Smith-Brown didn’t feature in Hearts’ 1-0 win over Kilmarnock.