Dens Park will be the venue for the exhibition match which will take place on Sunday 13 July 2014 and form part of the Blues' 2014/15 preparations.
Ferran Soriano, Manchester City Chief Executive Officer, said: “Manchester City is deeply proud of its historic ties with Scotland, having had many greats such as Willie Donachie, Paul Dickov and Colin Hendry wear the Club crest.
“The manager and players alike are extremely excited about visiting Dundee, to not only see our loyal fans, but also prepare for the new season against strong opposition who richly deserve their title as SPFL Championship champions.
“Dundee is a club steeped in history, so it’s sure to be a great game of football with a fantastic atmosphere for both sets of fans in Dens Park.”
Dundee FC Chief Executive Scot Gardiner, said: “We are thrilled to play against Manchester City in our opening game of next season and Manager Paul Hartley and his team will face a fantastic test of football.
"I’m particularly pleased for our fans and indeed football fans across Scotland who will have a rare chance to see Manuel Pelligrini’s side of superstars up close and personal at Dens Park."
Dundee FC Manager Paul Hartley, added: “To have such a challenge match against a team of the calibre of Manchester City at the beginning of next season shows exactly how big our club have been thinking recently. I look forward to matching our squad of players against Manchester City.”
This encounter marks the third friendly meeting between the clubs.
City last played Dundee in San Jose in 1986 in a match the Blues won 4-2 on penalties after a 1-1 draw in which Mark Lillis scored for the Blues.
The teams also met at Dens Park earlier in the same year and played out a 2-2 draw. Their first ever meeting was at Maine Road in 1965.
Information on how to buy match tickets will be made available in the coming week.