Paul Harsley's side beat League One Shrewsbury on penalties in the last-32 having topped Northern Group F in the previous stage of the competition.
It's been a fine run for our young charges against senior opposition.
Scunthorpe currently sit 16th in League Two.
Ticket information for the game at the Sands Venue Stadium will follow in due course.
Full EFL Cup third round draw:
Salford v Port Vale
Scunthorpe United v MANCHESTER CITY U21S
Tranmere v Leicester U21s
Fleetwood v Accrington
Walsall v Portsmouth
Bristol Rovers v Stevenage
Newport v MK Dons
Exeter v Ipswich