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The game will take place at the City Football Academy and be staged on Pitch 8 at the complex.
The winners will play either Middlesbrough or Derby County in the final.
Saturday's semi-final must be decided on the day, with extra-time and penalties if required.
For security reasons, spectators will now be limited to player families for the game. Seasoncards will not be accepted.
Gareth Taylor's Under-18s will travel to Bournemouth in the FA Youth Cup sixth round.
Manchester City Under-18s boss hails attitude of his players after 10-man Blues stage stirring FA Youth Cup fightback to overcome Derby Counyt