The defender has made 29 senior international appearances so far, and will be taking part in his first World Cup tournament in Qatar.
Ake has been at the centre of City's success so far this season, with his versatility proving crucial as the games come thick and fast.
He has made 13 appearances for Pep Guardiola's men ahead of the winter break, and is sure to be just as important in Louis van Gaal's side during the World Cup.
🦁 Our 26 Lions for the @FIFAWorldCup!#NothingLikeOranje | #FIFAWorldCup pic.twitter.com/EpkvaksBLW— OnsOranje (@OnsOranje) November 11, 2022
The Dutch failed to qualify for the 2018 edition of the tournament, but reached the semi-finals in 2014, and were runners up four years earlier.
And Ake will be hoping to go one step further this time round as the Netherlands look for their first World Cup triumph.
That journey begins against Senegal on Monday 21 November, with further group fixtures against Qatar and Ecuador to follow.
The defender is joined by two other Premier League players in the squad, those being Liverpool's Virgil van Dijk and Manchester United defender Tyrell Malacia.
Congratulations to Nathan on his inclusion in the Netherlands squad!
Justin Bijlow (Feyenoord)
Remko Pasveer (Ajax)
Andries Noppert (sc Heerenveen)
Virgil van Dijk (Liverpool)
Nathan Ake (Manchester City)
Daley Blind (Ajax)
Jurrien Timber (Ajax
Denzel Dumfries (Internazionale)
Stefan de Vrij (Internazionale)
Mathijs de Ligt (Bayern München)
Tyrell Malacia (Manchester United)
Jeremie Frimpong (Bayer Leverkusen)
Frenkie de Jong (FC Barcelona)
Steven Berghuis (Ajax)
Davy Klaassen (Ajax)
Teun Koopmeiners (Atalanta Bergamo)
Cody Gakpo (PSV)
Marten de Roon (Atalanta Bergamo)
Kenneth Taylor (Ajax)
Xavi Simons (PSV)
Memphis Depay (FC Barcelona)
Steven Bergwijn (Ajax)
Vincent Janssen (Royal Antwerp FC)
Luuk de Jong (PSV)
Noa Lang (Club Brugge)
Wout Weghorst (Beşiktaş JK)