The Dutch beat Austria 2-0 at the Johan Cruyff Arena and are now guaranteed a place in the knockout stages.
Just as in the opening game against Ukraine, Ake was introduced on 64 minutes for Daley Blind.
At that point, the Netherlands were leading through Memphis Depay’s 11th-minute penalty.
Ake, winning his 22nd cap, gave a typically industrious, energetic performance on the left of a back three.
He’d been on just three minutes when Denzel Dumfries doubled the lead with a swashbuckling run down the right and close range finish after Donyell Malen had raced clear and slid the ball across to the right wing-back who also scored against Ukraine.
The Dutch will wrap up their group stage fixtures against North Macedonia next Monday, with Ake hoping to win a starting berth against the side currently bottom of Group C.