City Elite Development Squad will kick off their 2013/14 Barclays U21 Premier League campaign at home to Fulham on Sunday 11 August.
The Premier League has also announced a change to the format of the competition for Patrick Vieira's first campaign in charge of the u21s.
Rather than the two phase, three-tiered system which was used last time, City will now play each of the 21 other eligible teams once, either home or away, in one-off fixtures which will run from August to the first week of April.
There will then be a knockout tournament at the end of April between the top four teams.
The team which finishes first will play fourth, leaving second to take on third in two semi-finals before the grand final which caps off the season.
Over the course of the year, the Blues will play Arsenal, Aston Villa, Blackburn Rovers, Bolton Wanderers, Chelsea, Everton, Fulham, Leicester City, Liverpool, Manchester United, Middlesbrough, Newcastle United, Norwich City, Reading, Southampton, Stoke City, Sunderland, Tottenham Hotspur, West Bromwich Albion, West Ham United and Wolverhampton Wanderers.
After kicking off the campaign at home to Fulham, City take on Spurs away on 19 August, before facing Everton in the Etihad Stadium on Bank Holiday Monday 26 August.
Sunderland will also visit the Etihad Stadium on March 28, while City’s youngsters will also experience games in first-team stadiums, such as St James Park, Carrow Road, Leicester’s King Power Stadium, Upton Park and The Hawthornes.
Vieira’s men play Manchester United away from home on Monday 27 January and finish off their league season away to Arsenal on Monday 7 April.
For all of the 2013/14 fixtures, please see our EDS fixtures page.