The famous symbol will adorn both sets of shirts in Houston on Thursday 20 July, as a mark of respect for those affected by the Manchester terrorist attack in May.
Following the game, which is the first meeting between the two clubs since the attack and the first Manchester derby to be played outside of the UK, both sets of match-worn shirts will be made available for fans to buy via auction.
All proceeds will go to the We Love Manchester Emergency Fund.
"Manchester continues to show great strength and sense of community since May’s devastating attack and the thoughts of everyone at Manchester City remain with those affected," said City CEO, Ferran Soriano, ahead of next week’s match.
"The worker bee symbolises everything that makes Manchester such a special city and our players will wear it on their shirts with immense pride, as a demonstration of solidarity with the Manchester community."
Denoting the industrial heritage of the city and its people, the worker bee has been an emblem since the Victorian era and was adopted into the coat of arms in 1842.
Its presence on both shirts comes after the launch of #ACITYUNITED, when both the Blue and Red half of the city came together to pledge a £1 million joint donation to the We Love Manchester Emergency Fund.
The money from the fund will help the families of the 22 people who died in the atrocity and the 64 people who were injured.
Ed Woodward, Manchester United Executive Chairman, echoed the sentiment of solidarity.
"The city of Manchester has shown great strength and unity since the tragic terror attack in May, showing the country and the world how special this city really is," he added.
"Having the worker bee on our shirts once again shows the community spirit of our city and football club and it is only natural that we continue to help all of those affected, through this fundraising and awareness, with millions of fans across the world watching this game."
Information on how to purchase the match-worn shirts will be announced via each club in the coming weeks.
For anyone who wishes to donate to the fund, please:
- Visit www.redcross.org.uk/manchester
- Visit www.justgiving.com
- Call 0300 456 4999
- Go to a British Red Cross shop