Having beaten Arsenal and drawn with Manchester United inside the club’s home this season, O’Brien and his Elite Development Squad teammates will face Leicester there on Friday night.
Manchester City's Premier League 2 side travelled to Sunderland looking for their third win of the campaign.
City's youngsters faced BMG at the Academy Stadium, just hours ahead of the Champions League match between the two clubs.
The Blues’ U19s face Borussia Monchengladbach at 3pm at the Academy Stadium on Tuesday in their first group game.
Ahead of tomorrow's first team match, City faced United in the Premier League at the Etihad Stadium.
The Blues have two wins from their opening three matches and will be looking to bounce back from their defeat at Reading at the end of August.
After two wins and a loss so far, the left-back sees the tie at the Etihad Stadium as the perfect opportunity to continue their impressive early season form.